期刊
FISHERIES SCIENCE
卷 72, 期 5, 页码 1004-1010出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1444-2906.2006.01249.x
关键词
differential display; film in situ zymography; Japanese flounder; matrix metalloproteinase 9 (MMP-9); Paralichthys olivaceus; skin; wound healing
类别
Fish, like mammals, have several stages of wound healing, including inflammation, reepithelialization, proliferation, organization, and differentiation. However, little is known about the molecular mechanisms of fish skin wound healing. In the present study, 23 polymerase chain reaction (PCR) bands were identified which appeared to be specifically expressed during wound healing by a simple differential display (DD) method. Sequences of cDNAs from these 14 bands were highly homologous to known genes. However, reverse transcriptase PCR (RT-PCR) analyses of these 14 bands revealed that only one of them, the gene for matrix metalloproteinase 9 (MMP-9), was significantly expressed in wounded skin, and that the others were probably false positives. MMPs are known to have gelatinolytic activity, and film in situ zymography detected strong gelatinolytic activity in the surface and dermis of wounded skin of flounder.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据