期刊
PLANT CELL
卷 18, 期 10, 页码 2567-2581出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.106.040931
关键词
-
资金
- NIGMS NIH HHS [GM-61306, P01 GM061306] Funding Source: Medline
We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/ Epidermal Growth Factor Receptor - Like Protein1 ( VSR- 1/ ATELP1) receptor during the formation of protein storage vacuoles in Arabidopsis thaliana embryos by means of high- pressure freezing/ freeze substitution, electron tomography, immunolabeling techniques, and subcellular fractionation. The storage proteins and their processing proteases are segregated from each other within the Golgi cisternae and packaged into separate vesicles. The storage protein - containing vesicles but not the processing enzyme - containing vesicles carry the VSR- 1/ ATELP1 receptor. Both types of secretory vesicles appear to fuse into a type of prevacuolar multivesicular body ( MVB). We have also determined that the proteolytic processing of the 2S albumins starts in the MVBs. We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据