期刊
JOURNAL OF DENTAL RESEARCH
卷 85, 期 10, 页码 900-904出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/154405910608501005
关键词
eugenol; trigeminal ganglion neurons; voltage-gated sodium channels
Although eugenol is widely used in dentistry, little is known about the molecular mechanisms responsible for its anesthetic properties. In addition to calcium channels, recently demonstrated by our group, there could be another molecular target for eugenol. Using a whole-cell patch-clamp technique, we investigated the effect of eugenol on voltage-gated sodium channel currents (I-Na) in rat dental primary afferent neurons identified by retrograde labeling with a fluorescent dye in maxillary molars. Eugenol inhibited action potentials and I-Na in both capsaicin-sensitive and capsaicin-insensitive neurons. The pre-treatment with capsazepine, a competitive antagonist of transient receptor potential vanilloid 1 (TRPV1), failed to block the inhibitory effect of eugenol on I-Na, suggesting no involvement of TRPV1. Two types of I-Na, tetrodotoxin (TTX)-resistant and TTX-sensitive I-Na, were inhibited by eugenol. Our results demonstrated that eugenol inhibits I-Na in a TRPV1-independent manner. We suggest that I-Na inhibition by eugenol contributes to its analgesic effect.
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