期刊
BIOMATERIALS
卷 27, 期 7, 页码 1152-1159出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.07.021
关键词
biodegradable; DNA delivery; layer-by-layer; poly-L-lysine; self-assembly
The layer-by-layer (LbL) self-assembly of poly-L-lysine (PLL) and deoxyribonucleic acid (DNA) was used to construct the enzymatic biodegradable multilayered films. The LbL build up of DNA multilayers was monitored by UV-vis spectrometry, and atomic force microscopy (AFM). AFM, UV-vis spectrometry and fluorescence spectrometry measurements indicated that 90% of DNA within the films was released almost linearly under 5 U mL(-1) alpha-chymotrypsin in PBS at 37 degrees C in 35 h. TEM and zeta potential experiments revealed that the released DNA molecules were condensed into the slight positive complexes with size from 20 to several hundred nanometers. The well-structured, easy processed enzymatic biodegradable multilayered film may have great potential for gene applications in tissue engineering, medical implants, etc. (c) 2005 Elsevier Ltd. All rights reserved.
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