期刊
NATURE METHODS
卷 3, 期 10, 页码 793-795出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth929
关键词
-
资金
- NIGMS NIH HHS [R01 GM068518, R01 GM068518-05] Funding Source: Medline
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据