期刊
PROTEOMICS
卷 6, 期 19, 页码 5169-5182出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200600209
关键词
bone marrow stromal cell; iTRAQ; leukemia; mesenchymal progenitor cell; mesenchymal stem cell
资金
- NCI NIH HHS [R21 CA092731] Funding Source: Medline
Bone marrow stromal. cells, also known as mesenchymal stem or progenitor cells, are thought to play a critical role in hematopoiesis. Because hematopoiesis is dysregulated in leukemic individuals, expression profiling in leukemic stromal cells might yield valuable insights into mechanisms of normal and dysregulated hematopoiesis. Here, proteomics was used to identify expressed proteins in normal and leukemic bone marrow stromal cells. Isobaric tags for relative and absolute quantification (iTRAQ) technology and MS/MS were used to identify and quantify proteins in bone marrow stromal. cells from two normal and two leukemic individuals. Approximately 900 distinct proteins were identified with > 95% confidence, 73 of which were differentially expressed in leukemic stromal cells. cDNA microarray analysis on Affymetrix oligonucleotide gene chips showed that transcripts correlated with approximately 90% of the expressed proteins. A novel integrated approach for mining and visualization of iTRAQ data is presented. The results provide an initial assessment of the proteome in human bone marrow stromal cells, laying the groundwork for comprehensive analysis of the proteome in these cells, and ultimately, for improved understanding of normal and dysregulated hematopoiesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据