期刊
JOURNAL OF NEUROCHEMISTRY
卷 99, 期 1, 页码 333-342出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1471-4159.2006.04073.x
关键词
alpha-synuclein; CD9; Multiple System Atrophy; neurodegeneration; Schwann cell; tubulin
P25 alpha/tubulin polymerization promoting protein (TPPP) is a brain specific phosphoprotein that displays microtubule bundling activity. In the mature brain, p25 alpha/TPPP distributes to oligodendrocytes and choroid plexus epithelium. We mapped the spatial and temporal distribution of p25 alpha/TPPP in the developing rat brain. Having localized its expression to neuronal tissue by Western blot analyses, the distribution of p25 alpha/TPPP to developing oligodendrocytes was confirmed using a specific antibody. In the pre-natal and post-natal brain, p25 alpha/TPPP was localized to the perinuclear cytoplasm of myelinating oligodendrocytes from embryonic (E) day E20 as verified from cellular co-localization with 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP). Oligodendrocyte progenitor cells and pre-myelinating oligodendrocytes identified by the expression of NG2 proteoglycan and CD9, respectively, both failed to contain p25 alpha/TPPP. In contrast, P25 alpha/TPPP co-localized with beta(IV)-tubulin from post-natal (p) day P10 suggesting that p25 alpha/TPPP plays an important role for tubulin-related transport in developing, myelinating oligodendrocytes.
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