期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 291, 期 4, 页码 F833-F839出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00474.2005
关键词
acid-base and fluid balance; mammalian collecting duct
资金
- NIDDK NIH HHS [DK-52935] Funding Source: Medline
Pendrin, encoded by Slc26a4, is a Cl-/HCO3- exchanger expressed in the apical region of type B and non-A, non-B intercalated cells, which regulates renal NaCl excretion. Dietary Cl- restriction upregulates total pendrin protein expression. Whether the subcellular expression of pendrin and whether the apparent vascular volume contraction observed in Slc26a4 null mice are Cl- dependent, but Na+ independent, is unknown. Thus the subcellular distribution of pendrin and its role in acid-base and fluid balance were explored using immunogold cytochemistry and balance studies of mice ingesting a NaCl-replete or a Na+-replete, Cl--restricted diet, achieved through substitution of NaCl with NaHCO3. Boundary length and apical plasma membrane pendrin label density each increased by similar to 60-70% in type B intercalated cells, but not in non-A, non-B cells, whereas cytoplasmic pendrin immunolabel increased similar to 60% in non-A, non-B intercalated cells, but not in type B cells. Following either NaCl restriction or Cl- restriction alone, Slc26a4 null mice excreted more Cl- and had a higher arterial pH than pair-fed wild-type mice. In conclusion, 1) following dietary Cl- restriction, apical plasma membrane pendrin immunolabel increases in type B intercalated cells, but not in non-A, non-B intercalated cells; and 2) pendrin participates in the regulation of renal Cl- excretion and arterial pH during dietary Cl- restriction.
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