Bone substitution materials on the basis of BONITmatrix® up-regulate mRNA expression of IGF1 and Col1a1

The aim of this study was to investigate the effects of BONITmatrix (R) and OSSA NOVA on the expression of growth factors and osteogenic differentiation. For this purpose, the mRNA expression of VEGF, IGF1, IGF2, collagen-1, collagen-2 and MMP8 was analysed in surgically created defects on the crania of adult male rats. Cranial samples were collected after implantation of BONITmatrix (R) or OSSA NOVA scaffolds for 4 weeks and determinations of gene expression were performed by quantitative RT-PCR. Real-time RT-PCR analyses showed a significantly higher expression of IGF1 in both groups treated with BONITmatrix (R) and OSSA NOVA compared to untreated controls, whereas type I collagen mRNA expression only increased in BONITmatrix (R) treated rats compared to controls. No changes in transcript expression of IGF2, VEGF, collagen-2 and MMP8 were detectable between the analysed groups. In conclusion, BONITmatrix (R) and OSSA NOVA stimulate the expression of growth factor IGF1, but only the granular dosage form is able to stimulate osteoblast differentiation. (C) 2011 Elsevier GmbH. All rights reserved.