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Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus agalactiae

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JOURNAL OF CLINICAL MICROBIOLOGY
卷 44, 期 10, 页码 3822-3825

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AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01232-06

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A 33-primer multiplex PCR-based reverse line blot (mPCR/RLB) assay was developed to identify Streptococcus agalactiae serotypes and surface protein antigen genes simultaneously. It was evaluated by using 551 clinical isolates. The mPCR/RLB assay was more sensitive than conventional serotyping, especially for protein antigen typing, but otherwise the results correlated well.

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