期刊
NATURE CHEMICAL BIOLOGY
卷 2, 期 10, 页码 529-530出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio816
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We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.
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