期刊
JOURNAL OF NEUROSCIENCE
卷 26, 期 40, 页码 10129-10140出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1202-06.2006
关键词
Alzheimer's disease; transgenic mice; A beta(42); amyloid plaques; intraneuronal A beta; neuron loss
资金
- NIA NIH HHS [P01 AG021184, R01 AG022560] Funding Source: Medline
Mutations in the genes for amyloid precursor protein (APP) and presenilins (PS1, PS2) increase production of beta-amyloid 42 (A beta(42)) and cause familial Alzheimer's disease (FAD). Transgenic mice that express FAD mutant APP and PS1 overproduceA beta(42) and exhibit amyloid plaque pathology similar to that found in AD, but most transgenic models develop plaques slowly. To accelerate plaque development and investigate the effects of very high cerebral A beta(42) levels, we generated APP/PS1 double transgenic mice that coexpress five FAD mutations (5XFAD mice) and additively increase A beta(42) production. 5XFAD mice generate A beta(42) almost exclusively and rapidly accumulate massive cerebral A beta(42) levels. Amyloid deposition (and gliosis) begins at 2 months and reaches a very large burden, especially in subiculum and deep cortical layers. Intraneuronal A beta(42) accumulates in 5XFAD brain starting at 1.5 months of age (before plaques form), is aggregated (as determined by thioflavin S staining), and occurs within neuron soma and neurites. Some amyloid deposits originate within morphologically abnormal neuron soma that contain intraneuronalA beta. Synaptic markers synaptophysin, syntaxin, and postsynaptic density-95 decrease with age in 5XFAD brain, and large pyramidal neurons in cortical layer 5 and subiculum are lost. In addition, levels of the activation subunit of cyclin-dependent kinase5, p25, are elevated significantly at 9 months in 5XFAD brain, although an upward trend is observed by 3 months of age, before significant neurodegeneration or neuron loss. Finally, 5XFAD mice have impaired memory in the Y-maze. Thus, 5XFAD mice rapidly recapitulate major features of AD amyloid pathology and may be useful models of intraneuronal A beta(42)-induced neurodegeneration and amyloid plaque formation.
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