期刊
JOURNAL OF CELL SCIENCE
卷 119, 期 20, 页码 4187-4198出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.03162
关键词
gap junctions; connexin43; teriparatide; bone anabolic agents; conditional gene deletion
类别
资金
- NIAMS NIH HHS [R01 AR041255] Funding Source: Medline
- NIDDK NIH HHS [P30 DK056341-05S2, P30 DK056341, P30 DK056341-06] Funding Source: Medline
Connexin43 (Cx43) is involved in bone development, but its role in adult bone homeostasis remains unknown. To overcome the postnatal lethality of Cx43 null mutation, we generated mice with selective osteoblast ablation of Cx43, obtained using a Cx43(fl) allele and a 2.3-kb fragment of the alpha(I)(I) collagen promoter to drive Cre in osteoblasts (ColCre). Conditionally osteoblast-deleted ColCre;Cx43(-/fl) mice show no malformations at birth, but develop low peak bone mass and remain osteopenic with age, exhibiting reduced bone formation and defective osteoblast function. By both radiodensitometry and histology, bone mineral content increased rapidly and progressively in adult Cx43(+/fl) mice after subcutaneous injection of parathyroid hormone (PTH), an effect significantly attenuated in ColCre;Cx43(-/fl) mice, with Cx43(-/fl) exhibiting an intermediate response. Attenuation of PTH anabolic action was associated with failure to increase mineral apposition rate in response to PTH in ColCre; Cx43(-/fl), despite an increased osteoblast number, suggesting a functional defect in Cx43-deficient bone-forming cells. In conclusion, lack of Cx43 in osteoblasts leads to suboptimal acquisition of peak bone mass, and hinders the bone anabolic effect of PTH. Cx43 represents a potential target for modulation of bone anabolism.
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