期刊
BLOOD
卷 108, 期 8, 页码 2596-2603出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2006-01-011817
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- NCI NIH HHS [P30 CA 21765, P01 CA 20180] Funding Source: Medline
- NHLBI NIH HHS [HL 079990] Funding Source: Medline
Botrocetin (bt)-facilitated binding of von Willebrand factor (VWF) to the platelet membrane glycoprotein (GP) Ib-IX-V complex on platelets in suspension initiates a signaling cascade that causes alpha IIb beta 3 activation and platelet aggregation. Previous work has demonstrated that bt/VWF-mediated agglutination activates alpha IIb beta 3 and elicits ATP secretion in a thromboxane A2 (TxA2)-dependent manner. The signaling that results in TxA2 production was shown to be initiated by Lyn, enhanced by Src, and propagated through Syk, SLP-76, PI3K, PLC gamma 2, and PKC. Here, we demonstrate that the signaling elicited by GPIb-mediated agglutination that results in TxA2 production is dependent on Bruton tyrosine kinase (Btk). The results demonstrate that Btk is downstream of Lyn, Syk, SLP-76, and PI3K; upstream of ERK1/2, PLCy2, and PKC; and greatly enhances Akt phosphorylation. The relationship(s), if any, between ERK1/2, PLC gamma 2, and PKC were not elucidated. The requirement for Btk and TxA2 receptor function in GPIb-dependent arterial thrombosis was confirmed in vivo by characterizing blood flow in ferric chloride-treated mouse carotid arteries. These results demonstrate that the Btk family kinase, Tec, cannot provide the function(s) missing because of the absence of Btk and that Btk is essential for both bt/VWF-mediated agglutination-induced TxA2 production and GPIb-dependent stable arterial thrombus formation in vivo.
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