期刊
BIOCHEMICAL JOURNAL
卷 399, 期 -, 页码 297-304出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20060873
关键词
Brahma-related gene 1 (Brg1); co-repressor erythroid; progenitor; histone deacetylase; SWI/SNF; transcriptional repression
资金
- NHLBI NIH HHS [R01 HL049118-13, R01 HL49118, R01 HL049118] Funding Source: Medline
SWI/SNF complexes are involved in both activation and repression of transcription. While one of two homologous ATPases, Brg 1 [Brm (Brahma)-related gene 1] or Brm, is required for their chromatin remodelling function, less is known about how these complexes are recruited to DNA. We recently established that a DNA-binding complex containing TAL1/SCL, E47, GATA-1, LMO2 and Ldb1 stimulates P4.2 (protein 4.2) transcription in erythroid progenitors via two E box-GATA elements in the gene's proximal promoter. We show here that the SWI/SNF protein Brg 1 is also associated with this complex and that both the E box and GATA DNA-binding sites in these elements are required for Brg 1 recruitment. Further, Brg 1 occupancy of the P4.2 promoter decreased with terminal erythroid differentiation in association with increased P4.2 transcription, while enforced expression of Brg 1 in murine erythroleukaemia cells reduced P4.2 gene expression. Overexpression of Brg 1 was associated with increased occupancy of the P4.2 promoter by the nuclear co-repressor mSin3A and HDAC2 (histone deacetylase 2) and with reduced histone H3 and H4 acetylation. Finally, a specific HDAC inhibitor attenuated Brg 1-directed repression of P4.2 promoter activity in transfected cells. These results provide insight into the mechanism by which,SWI/SNF proteins are recruited to promoters and suggest that transcription of P4.2, and most likely other genes, is actively repressed until the terminal differentiation of erythroid progenitors.
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