期刊
JOURNAL OF NEUROSCIENCE
卷 26, 期 42, 页码 10916-10924出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3269-06.2006
关键词
dendrite outgrowth; IP3 receptor; BDNF; Purkinje cell; granule cell; Ca2+ release
Here, we show that cultured Purkinje cells from inositol 1,4,5-trisphosphate receptor type 1 knock-out (IP(3)R1KO) mice exhibited abnormal dendritic morphology. Interestingly, despite the huge amount of IP(3)R1 expression in Purkinje cells, IP(3)R1 in granule cells, not in the Purkinje cells, was responsible for the shape of Purkinje cell dendrites. We also found that BDNF application rescued the dendritic abnormality of IP(3)R1KO Purkinje cells, and that the increase in BDNF expression in response to activation of AMPA receptor (AMPAR) and metabotropic glutamate receptor (mGluR) was impaired in IP(3)R1KO cerebellar granule cells. In addition, we observed abnormalities in the dendritic morphology of Purkinje cells and in the ultrastructure of parallel fiber-Purkinje cell (PF-PC) synapses in IP(3)R1KO mice in vivo. We concluded that activation of AMPAR and mGluR increases BDNF expression through IP(3)R1-mediated signaling in cerebellar granule cells, which contributes to the dendritic outgrowth of Purkinje cells intercellularly, possibly by modifying PF-PC synaptic efficacy.
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