期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2006.05.018
关键词
carvedilol enantiomers; HPLC; human whole blood; human plasma
A highly sensitive HPLC method for enantioselective determination of carvedilol inhuman whole blood and plasma was developed. Carvedilol and S-carazolol as an internal standard extracted from whole blood or plasma were separated using an enantioselective separation column (Chiralpak AD column; 2.0 circle divide x 250 mm) without any chiral derivatizations. The mobile phase was hexane:isopropanol:diethylamine (78:22:1, v/v). The excitation and emission wavelengths were set at 284 and 343 nm, respectively. The limits of quantification for the S(-)- and R(+)-carvedilol enantiomers in plasma and blood were both 0.5 ng/ml. Intra- and inter-day variations were less than 5.9%. As an application of the assay, concentrations of carvedilol enantiomer in plasma and blood samples from 15 patients treated with carvedilol for congestive heart failure were determined. (c) 2006 Elsevier B.V. All rights reserved.
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