期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1130, 期 2, 页码 175-181出版社
ELSEVIER
DOI: 10.1016/j.chroma.2006.04.010
关键词
endcapping; silica; monolithic; column; TMSI; HPLC
Following a bonding reaction with octadecyldimethyl-N,N-diethylaminosilane (ODS-DEA), a monolithic silica was subjected to consequential on-line endcapping reactions with hexamethyldisilazane (HMDS) or N-(trimethylsilyl)imidazole (TMSI) to compare the two reagents. Endcapping of monolithic silica C-18 columns prepared by a manufacturer was also studied. After endcapping with TMSI in acetonitrile (ACN), all columns provided better performance for hydrogen-bonding compounds and basic compounds than C-18 phases endcapped with HMDS. TMSI endcapping of commercial Chromolith Performance columns also gave improved performance. The results indicate that one can significantly improve the performance of monolithic silica C-18 columns for basic compounds by a simple on-column reaction with TMSI in ACN at a relative low temperature. (c) 2006 Elsevier B.V. All rights reserved.
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