The dissociation of inorganic phosphate (Pi) following GTP hydrolysis is a key step determining the functional state of many GTPases. Here, the timing of Pi release from elongation factor Tu (EF-Tu) and its implications for the function of EF-Tu on the ribosome were studied by rapid kinetic techniques. It was found that Pi release from EF-Tu is > 20-fold slower than GTP cleavage and limits the rate of the conformational switch of EF-Tu from the GTP- to the GDP-bound form. The point mutation Gly94Ala in the switch 2 region of EF-Tu abolished the delay in Pi release, suggesting that Pi release is controlled by the mobility of the switch 2 region with Gly94 acting as a pivot. The rate of Pi release or the conformational switch of EF-Tu does not affect the selection of aminoacyl-tRNA on the ribosome. Rather, the slow Pi release may be a consequence of the tight interaction of the switch regions of EF-Tu with the gamma-phosphate and the ribosome in the GTPase activated state of the factor.
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