期刊
ANALYTICA CHIMICA ACTA
卷 580, 期 1, 页码 1-8出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2006.07.063
关键词
fumonisin B-1; enhanced chemiluminescence; enzyme-linked immunosorbent assay (ELISA); food
An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibody with enhanced chemiluminescent (ECL) detection of fumonisin B, (FBI) in food samples has been developed. Assay conditions, including concentrations of antibody and enzyme conjugate, competition time and so on, were optimized. The effects of pH and two different organic solvents were investigated. The optimized ECL-ELISA system allowed FBI determination in a linear working range of 0.14-0.9 mu g L-1 with IC50 value of 0.32 mu g L-1 and a limit of detection of 0.09 mu g L-1. The ECL-ELISA was about 10 times more sensitive and about 30% time less than that of colorimetric ELISA using the same antibody and HRP-conjugate. Good recoveries with spiked food samples were obtained, and the results correlated well with those obtained using conventional direct competition ELISA assay and HPLC method, which indicated that ECL-ELISA was capable of being applied for the specific detection and routine monitoring of FBI in food samples. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据