期刊
NEUROSCIENCE
卷 142, 期 3, 页码 717-725出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2006.06.050
关键词
migration; neurogenesis; traumatic brain injury; subependymal zone; fate; mouse
资金
- NIA NIH HHS [R01 NS/AG42253-01] Funding Source: Medline
- NINDS NIH HHS [K08 NS053529, K08 NS053529-01] Funding Source: Medline
Subventricular zone (SVZ) cells emigrate toward brain injury but relatively few survive. Thus, if they are to be used for repair, ex vivo expansion and autologous transplantation of SVZ cells may be necessary. Since it is unclear how brain injury alters SVZ cell culture, we studied neurosphere formation, differentiation, and migration, after cortical lesions. The number of neurosphere forming cells from lesioned mice was comparable to controls. Also, the proportion of astrocytes and neurons generated in vitro remained unchanged after cortical lesions. Cell emigration from neurospheres was characterized by increased cell-cell contact after injury in adults and neonates. However, neither molecules implicated in SVZ migration nor the extent of migration changed after injury. Thus, neurospheres can be successfully cultured after extensive brain damage, and they are remarkably stable in vitro, suggesting suitability for ex vivo expansion and autologous transplantation. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
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