Bioactive compounds from the seed fungus Menisporopsis theobromae BCC 3975

Eight new compounds (2-9), together with a known dithiodiketopiperazine ( 1), were isolated from the seed fungus Menisporopsis theobromae BCC 3975. The structures of these substances were elucidated by analyses of spectroscopic data. Compounds 1 and 4 exhibited moderate cytotoxicity against BC-1 cell lines with IC50 values of 29.2 and 57.4 mu M, respectively. Cytotoxicity of 1, 2, 4, and 9 against the NCI-H187 cell line showed respective IC50 values of 22.9, 20.3, 1.8, and 56.6 mu M. Compounds 2 and 4 exhibited antimalarial activity with IC50 values of 2.95 and 28.8 mu M, respectively. Substances 1, 4, 7, 8, and 9 possessed weak antimycobacterial activity ( MIC 154.8-952.3 mu M), while compounds 2 and 3 showed potent antimycobacterial activity with respective MIC values of 1.24 and 7.14 mu M.