期刊
BIOCATALYSIS AND BIOTRANSFORMATION
卷 24, 期 6, 页码 449-457出版社
INFORMA HEALTHCARE
DOI: 10.1080/10242420601068668
关键词
multistep synthesis; reaction modelling; transketolase; omega-transaminase; one-pot synthesis
The most attractive, as well as challenging, multistep organic syntheses would preferably be carried out in a single reactor, as a one-pot synthesis. For biocatalytic syntheses, multistep reactions in one-pot mode bring a number of advantages, while at the same time raising unique challenges such as the compatibility of different biocatalysts. In this paper, we have developed a transketolase-transaminase (TK-TAm) two-step one-pot aminotriol synthesis reaction model, which integrates reaction kinetic models with process characterization (consisting of component degradation as a function of pH and concentration, aldehyde toxicity towards the enzyme, and ketol donor and acceptor side-reactions with TAm). Based on the analysis of the effect of the TAm/TK activity ratio on product yield, simulations provided guidance for further process and biocatalyst development.
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