SC2: A novel process for manufacturing multipurpose high-density chemical microarrays

Various formats of miniaturized arrays of cellulose-bound synthetic probe molecules to suit a broad range of technical and assay configurations have been produced by a novel process which we call spotting compound-support conjugates, SC2. This process involves parallel chemical assembly of the probe molecules by SPOT-synthesis on a special cellulose membrane, post-synthesis separation of the tethered compound spots, solubilization of the cellulose matrix and spotting of the soluble cellulose-probe conjugates onto various types of planar surfaces. The performance of such arrays are demonstrated using a set of synthetic peptide analogues, assayed with a monoclonal antibody, as well as utilizing a small molecule library of diketopiperazines assayed with fluorescently labelled calmodulin.