期刊
PLANT PHYSIOLOGY
卷 142, 期 3, 页码 993-1003出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.106.085043
关键词
-
The plastid genome is transcribed by three different RNA polymerases, one is called plastid- encoded RNA polymerase (PEP) and two are called nucleus- encoded RNA polymerases (NEPs). PEP transcribes preferentially photosynthesis- related genes in mature chloroplasts while NEP transcribes preferentially housekeeping genes during early phases of plant development, and it was generally thought that during plastid differentiation the building up of the NEP transcription system precedes the building up of the PEP transcription system. We have now analyzed in detail the establishment of the two different transcription systems, NEP and PEP, during germination and early seedling development on the mRNA and protein level. Experiments have been performed with two different plant species, Arabidopsis (Arabidopsis thaliana) and spinach (Spinacia oleracea). Results show that the building up of the two different transcription systems is different in the two species. However, in both species NEP as well as PEP are already present in seeds, and results using Tagetin as a specific inhibitor of PEP activity demonstrate that PEP is important for efficient germination, i.e. PEP is already active in not yet photosynthetically active seed plastids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据