期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 80, 期 5, 页码 1156-1164出版社
WILEY
DOI: 10.1189/jlb.0206125
关键词
vascular injury; chemokines; CX3CR1; neurotoxicity; neurogeneration
资金
- NIDA NIH HHS [DA016549] Funding Source: Medline
- NINDS NIH HHS [NS35734] Funding Source: Medline
The CD16(+) subset of peripheral blood monocytes (Mo) is expanded dramatically during inflammatory conditions including sepsis, HIV-1 infection, and cancer. CD16(+) express high levels of CX3CR1, which mediates arrest onto CX3CL1-expressing endothelial cells (EC) under flow conditions. In contrast, attachment of CD16(-) Mo onto cytokine-activated EC is independent of CX3CL1. Here, we investigate the ability of CD16(+) and CD16(-) Mo to produce proinflammatory cytokines upon interaction with CX3CL1-expressing HUVEC. We demonstrate that CD16(+) but not CD16(-) Mo produce high levels of IL-6, CCL2, and matrix metalloproteinase (MMP)-9 when cocultured with TNF/IFN-gamma-activated HUVEC or nonactivated HUVEC expressing CX3CL1. Furthermore, supernatants from Mo cocultured with cytokine-activated HUVEC induce neuronal death in vitro. These results suggest that membrane-bound CX3CL1 stimulates production of IL-6, CCL2, and MMP-9 by CD16(+) Mo, likely via engagement of CX3CR1. Thus, expansion of CD16(+) Mo and their accumulation onto CX3CL1-expressing EC may result in recruitment of Mo and T cell subsets at sites of inflammation in response to CCL2, IL-6-induced cell activation and/or differentiation, and MMP-9-mediated vascular and tissue injury. J. Leukoc. Biol. 80: 1156-1164; 2006.
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