期刊
PHILOSOPHICAL MAGAZINE
卷 86, 期 33-35, 页码 5705-5714出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786430600599757
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The influence of various experimental conditions on the elastic modulus and hardness of human intertubular dentin was studied using instrumented nanoindentation at room temperature. The conditions studied were: dry (chemically dehydrated) versus wet (prepared and nanoindented under Hank's balanced salt solution (HBSS)), the influence of long-term storage under HBSS at ca. 4 degrees C and the influence of deep-freezing under dry and wet conditions. The reversibility of chemical dehydration and the consequences of multiple deep-freezing cycles were also investigated. Two premolars of a 12-year-old human male were chosen for evaluation. The absolute values of the mechanical properties differed by a factor of similar to 2 and similar to 3 between dry and wet states, whereas trends were conserved. Deep-freezing wet dentin samples weakened their mechanical properties by 20 - 28%, whereas dehydrated samples suffered no damage. This may be due to mechanical damage via the volume increase in water freezing inside the tubules.
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