期刊
STEM CELLS
卷 24, 期 11, 页码 2346-2354出版社
ALPHAMED PRESS
DOI: 10.1634/stemcells.2005-0605
关键词
laminin-5/laminin-332; mesenchymal stem cells; cell growth; chondrogenesis; osteogenesis
Laminin-5 (laminin-332) is an important basement membrane protein that regulates cell attachment and motility. Recent studies have shown that laminin-5 is expressed in human mesenchymal stem cells (MSCs) in culture and that the laminin gamma 2 chain is transiently expressed in chondrocytes during development. These studies suggest that laminin-5 may be involved in the regulation of chondrogenic differentiation of MSCs. In this study, we examined a possible role of laminin-5 in the proliferation and differentiation of human MSCs. When MSCs were incubated in the presence of a coated or soluble form of laminin-5 in a growth medium, they proliferated more rapidly than nontreated cells, keeping their differentiation potential. On the other hand, laminin-5 potently suppressed the chondrogenic differentiation of MSCs. These activities were mediated mainly by integrin alpha 3 beta 1. However, laminin-5 had no effect on the osteogenic differentiation of MSCs. These results suggest that laminin-5 may contribute to the development of bone tissues by promoting the proliferation and by suppressing the chondrogenic differentiation of MSCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据