期刊
BIOPHYSICAL JOURNAL
卷 91, 期 9, 页码 3313-3326出版社
CELL PRESS
DOI: 10.1529/biophysj.106.091421
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资金
- NIAID NIH HHS [R37 AI030557] Funding Source: Medline
- NIGMS NIH HHS [P01 GM072694, GM072694] Funding Source: Medline
Cell membranes have complex lipid compositions, including an asymmetric distribution of phospholipids between the opposing leaflets of the bilayer. Although it has been demonstrated that the lipid composition of the outer lea. et of the plasma membrane is sufficient for the formation of raft-like liquid-ordered (l(o)) phase domains, the influence that such domains may have on the lipids and proteins of the inner lea. et remains unknown. We used tethered polymer supports and a combined Langmuir-Blodgett/vesicle fusion (LB/VF) technique to build asymmetric planar bilayers that mimic plasma membrane asymmetry in many ways. We show that directly supported LB monolayers containing cholesterol-rich l(o) phases are inherently unstable when exposed to water or vesicle suspensions. However, tethering the LB monolayer to the solid support with the lipid-anchored polymer 1,2-dimyristoyl phophatidylethanolamine-N-[poly(ethylene glycol)-triethoxysilane]significantly improves stability and allows for the formation of complex planar-supported bilayers that retain > 90% asymmetry for 1-2 h. We developed a single molecule tracking (SPT) system for the study of lipid diffusion in asymmetric bilayers with coexisting liquid phases. SPT allowed us to study in detail the diffusion of individual lipids inside, outside, or directly opposed to lo phase domains. We show here that lo phase domains in one monolayer of an asymmetric bilayer do not induce the formation of domains in the opposite lea. et when this lea. et is composed of palmitoyl-oleoyl phosphatidylcholine and cholesterol but do induce domains when this lea. et is composed of porcine brain phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and cholesterol. The diffusion of lipids is similar in l(o) and liquid-disordered phase domains and is not affected by transbilayer coupling, indicating that lateral and transverse lipid interactions that give rise to the domain structure are weak in the biological lipid mixtures that were employed in this work.
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