Bacillus infantis sp nov and Bacillus idriensis sp nov., isolated from a patient with neonatal sepsis

Two Gram-positive bacilli, designated as strains SMC 4352-1(T) and SMC 4352-2(T), were isolated sequentially from the blood of a newborn child with sepsis. They could not be identified by using conventional clinical microbiological methods. 16S rRNA gene sequencing and phylogenetic analysis revealed that both strains belonged to the genus Bacillus but clearly diverged from known Bacillus species. Strain SMC 4352-1(T) and strain SMC 4352-2(T) were found to be closely related to Bacillus firmus NCIMB 9366 T (98.2% sequence similarity) and Bacillus cibi JG-30(T) (97.1% sequence similarity), respectively. They also displayed low DNA-DNA reassociation values (less than 40%) with respect to the most closely related Bacillus species. On the basis of their polyphasic characteristics, strain SMC 4352-1(T) and strain SMC 4352-2(T) represent two novel species of the genus Bacillus, for which the names Bacillus infantis sp. nov. (type strain SMC 4352-1(T) = KCCM 90025(T) = JCM 13438(T)) and Bacillus idriensis sp. nov. (type strain SMC 4352-2(T) = KCCM 90024(T) = JCM 13437(T)) are proposed.