期刊
CALCIFIED TISSUE INTERNATIONAL
卷 79, 期 5, 页码 301-309出版社
SPRINGER
DOI: 10.1007/s00223-006-0082-7
关键词
hypoxia; osteoclast differentiation; giant osteoclast; cat
资金
- Wellcome Trust Funding Source: Medline
Dental disease due to osteoclast (OC) overactivity reaches epidemic proportions in older domestic cats and has also been reported in wild cats. Feline odontoclastic resorptive lesions (FORL) involve extensive resorption of the tooth, leaving it liable to root fracture and subsequent loss. The etiopathogenesis of FORL remains unclear. Here, we explore the hypothesis that FORL is associated with hypoxia in the oral microenvironment, leading to increased OC activity. To investigate this, we developed a method of generating OCs from cat blood. Reducing O-2 from 20% to 2% increased the mean area of OC eightfold from 0.01 to 0.08 mm(2). In hypoxic cultures, very large OCs containing several hundred nuclei were evident ( reaching a maximum size of similar to 14 mm(2)). Cultures exposed to 2% O-2 exhibited an increase of similar to 13-fold in the area of bone slices covered by resorption lacunae. In line with this finding, there was a significant increase in cells differentiating under hypoxic conditions, reflected in increased expression of cathepsin K and proton pump enzymes. In conclusion, these results demonstrate that oxygen tension is a major regulator of OC formation in the cat. However, in this species, hypoxia induces the formation of giant OCs, which can be so large as to be visible with the naked eye and yet also actively resorb. This suggests that local hypoxia is likely to play a key role in the pathogenesis of FORL and other inflammatory conditions that are associated with bone resorption in cats.
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