We found the colorimetric reaction of Tiron (1,2-dihydroxybenzene-3,5-disulfonic acid) and molybdate suitable for optical quantification of chemical reaction during fluid-fluid mixing in laboratory chambers. This reaction consists of two colorless reagents that mix to rapidly form colored, stable, soluble products. These products can be digitally imaged and quantified using light absorbance to study fluid-fluid mixing. Here we provide a model and equilibrium constants for the relevant complexation reactions. We also provide methods for relating light absorbance to product concentrations. Practical implementation issues of this reaction are discussed and an example of imaged absorbances for fluid-fluid mixing in heterogeneous porous media is given.
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