期刊
JOURNAL OF DAIRY RESEARCH
卷 73, 期 4, 页码 454-463出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0022029906001853
关键词
high pressure homogenisation; raw milk; alkaline phosphatase; microbial inactivation; fat globule size distribution
Raw whole milk of high microbial quality (<= 4 x 10(4) cfu/ml) was processed using a similar to 15 l/h homogeniser with a high pressure (HP) valve immediately followed by cooling heat exchangers. The effects of homogenisation between 100 and 300 MPa (HP valve) with an initial milk temperature T-in = 4 degrees C or 24 degrees C was investigated on the inactivation of: (i) endogenous alkaline phosphatase (ALP); (ii) endogenous milk flora and (iii) two Gram positive (Listeria innocua and Micrococcus luteus) and one Gram negative (Pseudomonas fluorescens) strains inoculated into milk. Temperatures T-1 and T-2 measured before and immediately after the HP valve, and fat globule size distributions were also determined. ALP activity slightly decreased after homogenisation above 250 MPa when T-in = 4 degrees C (corresponding T-2 > 58 degrees C), but markedly decreased above 200 MPa when T-in = 24 degrees C (T-2 > 60 degrees C). In contrastto inactivation induced by continuous short-time thermal treatments, ALP inactivation induced by HP homogenisation was clearly due to mechanical forces (shear, cavitation and/or impact) in the HP valve and not to the short (<< 1 s) residence time at temperature T-2 in the same valve. Inactivation of the three exogenous microorganisms led to similar conclusions. Homogenisation at 250 MPa or 300 MPa (T-in = 24 degrees C) induced a 2 - 3 log cycle reduction of the total enclogenous milk flora and a 1.5 - 1.8 log cycle reduction of inoculated List. innocua. Higher reduction ratios (2 - 4 log cycles) were obtained for the two other microorganisms. The highest levels of ALP inactivation corresponded to the highest extents of microbial reduction. Running the milk twice or three times through the homogeniser (recycling), keeping temperature T-1 approximate to 29 degrees C and pressure = 200 MPa, increased homogenisation efficiency.
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