期刊
NATURE METHODS
卷 3, 期 11, 页码 891-893出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH934
关键词
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资金
- NIGMS NIH HHS [R01 GM065367] Funding Source: Medline
Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.
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