期刊
ANATOMY AND EMBRYOLOGY
卷 211, 期 6, 页码 631-638出版社
SPRINGER
DOI: 10.1007/s00429-006-0125-x
关键词
heat shock protein; embryogenesis; transgenic mice; EGFP; insulator
The Hsp70.2/Hst70 gene is a unique member of the 70 kDa heat shock proteins multigene family whose activity is regulated developmentally; in adult mice and rats its expression is restricted mostly to meiotic and postmeiotic male germ cells. In aim to analyze activity of the Hsp70.2/Hst70 promoter in developing embryos we have constructed transgenic mice expressing EGFP reporter gene under control of the rat Hst70 promoter. The appearance of EGFP fluorescence coincides with series of major developmental events, such as extra-embryonic membranes formation, axial rotation, formation of neural tube and the primordium of central nervous system, formation of differentiated somites, extensive remodeling of the heart, development of fingers and toes, and sensory organs formation. Activity of the Hst70 promoter localizes mostly inside nervous system indicating the role of Hsp70.2/Hst70 gene in development of this system.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据