期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 13, 期 11, 页码 965-972出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb1158
关键词
-
资金
- MRC [MC_U105459896, MC_U105184326] Funding Source: UKRI
- Medical Research Council [MC_U105459896, MC_U105184326] Funding Source: researchfish
- Medical Research Council [MC_U105184326, MC_U105459896] Funding Source: Medline
- Wellcome Trust [068713] Funding Source: Medline
The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsK alpha beta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsK gamma through its interaction with KOPS. By solving the FtsK gamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsK gamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据