期刊
CHINESE SCIENCE BULLETIN
卷 51, 期 21, 页码 2586-2594出版社
SCIENCE PRESS
DOI: 10.1007/s11434-006-2173-2
关键词
infrared camera; guard cell; genetic analysis; signal transduction
In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appropriate state. However, many signaling components in guard cell signaling remain elusive. In our laboratory, a tool for non-invasive remote infrared thermal images was used to screen an ethyl methane sulfonate-mutagenized population for Arabidopsis stomatal response mutants under multiple stresses (ABA, H2O2, CO2, etc.). More than forty hot or cold mutants were isolated (above or below 0.5 degrees C in contrast to normal plantlets). Identification and primary genetic analysis of these mutants show that they are monogenic recessive mutations and there exist distinct difference in stomata apertures compared to wild type. These mutants in response to various environmental stresses and hormones were comprehensively investigated, which enables us to further understand the cross-talk in different signal transduction pathways.
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