期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 57, 期 14, 页码 3781-3791出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erl191
关键词
Blumeria graminis; cell wall softening; hydrogen peroxide; HvRBOH; penetration; resistance; superoxide (O-2(-)); susceptibility
Reactive oxygen intermediates (ROI) are closely related to defence reactions of plants against pathogens. A prominent role in the production of ROI has been attributed to the plant respiratory burst oxidase homologues (RBOH) of the human phagocyte GP91(phox). A barley RBOH, which encodes a putative superoxide (O-2(.-)) producing NADPH oxidase, is described here. Histochemical analysis of the barley-Blumeria graminis f. sp. hordei (Bgh) interaction showed that O-2(.-) is produced locally at the site of penetration. In contrast, hydrogen peroxide (H2O2) is produced in non-penetrated cell wall appositions. A barley RBOHA cDNA was isolated and a minor induction of expression of RBOHA was observed during the interactions of barley with Bgh. Transient RNA interference-mediated gene silencing of HvRBOHA during the penetration process of Bgh led to an increase of basal penetration resistance. The results support a potential role of HvRBOHA in cellular accessibility to Blumeria graminis.
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