期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 349, 期 4, 页码 1294-1300出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.08.163
关键词
14-3-3 protein; alpha-synuclein; DJ-1; dopamine toxicity; HSP70; human neuroblastoma; oxidative stress; Parkinson's disease; two-dimensional electrophoresis
Dopaminergic human neuroblastoma SH-SY5Y cells were stably transformed to increase expression of alpha-synuclein, a Parkinson's disease-related protein. Transformed cells were more resistant to oxidative insults, showing a cytoprotective role of alpha-synuclein. The expression of redox chaperonins (DJ-1, HSP70, and 14-3-3) was evaluated by Western blotting. Expression of alpha-synuclein reduced HSP70 levels even in the presence of dopamine, with a twofold increase of DJ-1 in the absence of oxidants. DJ-1 is significantly reduced by dopamine, and even more by dopamine and Cu(II). Increased alpha-synuclein expression did not affect 14-3-3, although dopamine increased its level by 60% in wild-type cells. alpha-Synuclein not only upregulated DJ-1, but also shifted all DJ-1 forms to a single spot at pI = 5.7 not observed in wild-type cells. Dopamine gradually restored the distribution of DJ-I forms to a situation similar to wildtype cells, with the form at pI = 6.1 progressively enriched under oxidative conditions. (c) 2006 Elsevier Inc. All rights reserved.
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