期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1133, 期 1-2, 页码 35-40出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.08.047
关键词
single drop liquid-equid-liquid microextraction; urine; drug analysis; methamphetamine; amphetamine
Single drop liquid-liquid-liquid microextraction (LLLME) combined with high performance liquid chromatography (HPLC)-UV detection was investigated for the determination of a popular drug of abuse, methamphetamine (MAP), and its major metabolite, amphetamine (AP), in urine samples. The target compounds were extracted from NaOH modified sample solution to a thin layer of organic solvent membrane, and back-extracted to an acidic acceptor drop suspended on the tip of a 50-mu L HPLC syringe in the aforementioned organic layer. This syringe was also used for direct injection after extraction. Factors affecting extraction efficiency were studied. At optimal conditions, the overall enrichment factor (EF) was 500-fold for AP and 730-fold for MAP, respectively. The method exhibited a wide linear range (1.0-1500 mu g/L), low detection limit (0.5 mu g/L), and good repeatability (RSD < 5.0%) for both analytes. The feasibility of the method was demonstrated by the analysis of human urine samples. (c) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据