期刊
VACCINE
卷 24, 期 44-46, 页码 6647-6650出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2006.06.001
关键词
viral RNAs; trans-complementation; RNA polymerase II; packaging; vaccine; influenza A virus
The incorporation of the eight gene segments of influenza A virus in virions, either through a random or a specific mechanism, has been under debate for many years. Using reverse genetics techniques and trans-complementation of viral proteins, we showed that the production of viruses containing seven gene segments is very inefficient, which can be overcome by providing the 8th gene segment in a non-functional form. We conclude that sequences in the 5' and 3' ends of the polymerase gene segments contain packaging signals. Our methods will facilitate the further mapping of the packaging signals of influenza A virus. (c) 2006 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据