期刊
BIOCONJUGATE CHEMISTRY
卷 17, 期 6, 页码 1373-1375出版社
AMER CHEMICAL SOC
DOI: 10.1021/bc0601018
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A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using click chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of enzymatic activity. The products have been characterized by gel electrophoresis and a fluorometric lipase activity assay. It is estimated that the equivalent of approximately seven fully active lipase molecules are attached to each nanoparticle.
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