期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 32, 期 2, 页码 93-99出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2006.09.007
关键词
1,3-Propanediol; bioconversion; immobilized cells; NaCS/PDMDAAC (sodium cellulose sulfate/poly-dimethyl-diallyl-ammonium chloride) microcapsules; fixed-bed bioreactor; glycerol
Klebsiella pneumoniae was encapsulated in the NaCS/PDMDAAC (sodium cellulose sulfate/poly-dimethyl-diallyi-ammonium chloride) microcapsule to produce 1,3-propanediol (PDO) from glycerol. Fermentations including batch culture, fed-batch culture and continuous culture were carried out in shake flasks and a fixed-bed bioreactor. Results showed that the NaCS/PDMDAAC microcapsule has desirable properties - such as biomass enrichment in the microcapsule (6.84 g/L capsule, 2.6-fold higher than that of free cell culture), enhanced substrate tolerance and stable activity of cells, operation convenience for continuous culture - for the microbial production of 1,3-propanediol. A 1,3-propanediol concentration of 63.1 g/L with a yield of 0.65 mol PDO/mol glycerol was obtained in the batch culture. The encapsulated cells remained its activity for 67 h in the fed-batch culture and produced 1,3-propanediol at a concentration of 51.86 g/L. In the continuous culture, 1,3-propanediol was provided at a productivity of 4.49 g/L h, a concentration of 13.6 g/L, and a yield of 0.43 mol PDO/mol glycerol at a dilution rate of 0.33 h(-1). (c) 2006 Elsevier B.V. All rights reserved.
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