期刊
EMBO JOURNAL
卷 25, 期 22, 页码 5358-5371出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.emboj.7601392
关键词
DNA; mitochondria; mtDNA; replication; vertebrate
资金
- MRC [MC_U105663140] Funding Source: UKRI
- Medical Research Council [MC_U105663140] Funding Source: researchfish
- Medical Research Council [MC_U105663140] Funding Source: Medline
Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 50 ends of DNA, initiation of RITOLS replication occurs in the major noncoding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin.
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