Manganese-enhanced 3D MRI of established and disrupted synaptic activity in the developing insect brain in vivo

The antennal lobe of the sphinx moth Manduca sexta serves as a model for the development of the olfactory system. Here, the establishment of the glomerular synaptic network formed by the olfactory receptor axons and antenna] lobe neurons at pupal stage P12 was followed by transection of the right antenna and - within 24 h - by injection of MnCl2 into the hemolymph. In vivo 3D MRI at 100 and 60 mu m isotropic resolution was then performed at P13 to P17. Whereas the left antennal lobe revealed a pronounced increase of the signal-to-noise ratio (SNR) reflecting normal synaptic activity, the observation of only a small SNR increase within the right antennal lobe indicated the disruption of pertinent activity after antennal transection. The accumulation of manganese in the intact antennal system became observable within 3 h and lasted for at least 2 days after injection. Intra-individual comparisons between the right and left side yielded a statistically significant differential SNR increase in the left antennal lobe. Because such an effect was not observed in younger animals studied at pupal stages P10/P11, the MRI findings confirm the development of functional synapses in the antennal lobe of Manduca sexta by P13. (c) 2006 Elsevier B.V. All rights reserved.