期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 281, 期 47, 页码 36124-36131出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M604152200
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资金
- NCI NIH HHS [P01CA097189, R01 CA85619, R01CA82259, K01CA104079] Funding Source: Medline
- NICHD NIH HHS [R01HD047470] Funding Source: Medline
The E2F family of transcription factors is believed to have an essential role in the control of cellular proliferation by regulating the transcription of genes involved in cell cycle progression. Previous work has demonstrated that the targeted inactivation of E2f1, E2f2, and E2f3 results in elevated p21(CIP1) protein levels, loss of E2F target gene expression, and cell cycle arrest at G(1)/S and G(2/)M, suggesting a strict requirement for these E2Fs in the control of normal cellular proliferation. We now demonstrate that E2f1, E2f2, and E2f3 are also required for oncogene-mediated transformation of mouse embryonic fibroblasts. Analysis of synchronized populations of mouse embryonic fibroblasts revealed that the inactivation of p21CIP1 restores the ability of E2f1-3-deficient cells to enter and transit through G(1)/S ( but not G(2)/M). In contrast, loss of p53 restored the ability of these cells to progress through both G(1)/S and mitosis, leading to their continued proliferation. The inactivation of p53 ( but not p21(CIP1)) rendered E2f1-3-deficient cells sensitive to transformation and tumorigenesis. These results suggest that the negative regulation of the p53-p21CIP1 axis by the E2F1-3 factors is critical for cell cycle progression and cellular transformation.
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