期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 350, 期 3, 页码 557-561出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.09.079
关键词
bone marrow transplantation; beta-galactosidase; fracture repair; mouse; inflammation; osteoblast; osteocyte; chondrocyte; osteoclast
资金
- NIA NIH HHS [R01 AG23218-01] Funding Source: Medline
- NIDCR NIH HHS [R01 DE012462, R03 DE16701] Funding Source: Medline
The bone marrow is believed to play important roles during fracture healing such as providing progenitor cells for inflammation, matrix remodeling.. and cartilage and bone formation. Given the complex nature of bone repair, it remains difficult to distinguish the contributions of various cell types. Here we describe a mouse model based on bone marrow transplantation and genetic labeling to track cells originating from bone marrow during fracture healing. Following lethal irradiation and engraftment of bone marrow expressing the LacZ transgene constitutively, wild type mice underwent tibial fracture. Donor bone marrow-derived cells, which originated from the hematopoietic compartment, did not participate in the chondrogenic and osteogenic lineages during fracture healing. Instead, the donor bone marrow contributed to inflammatory and bone resorbing cells. This model can be exploited in the future to investigate the role of inflammation and matrix remodeling during bone repair, independent from osteogenesis and chondrogenesis. (c) 2006 Elsevier Inc. All rights reserved.
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