期刊
FEBS LETTERS
卷 580, 期 27, 页码 6322-6328出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.10.040
关键词
guanine nucleotide exchange factor; biacore; catalytic region; mutagenesis; intramolecular interaction
The Ras-specific guanine nucleotide exchange region of hSos1 consists of two consecutive domains: the catalytic core (residues 742-1024) contains all residues binding to Ras, including the catalytic hairpin, and an upstream REM domain (residues 553-741), so called because it contains an evolutionary conserved Ras Exchange Motif (REM). We functionally define the boundaries of the REM domain through a combination of in vivo and in vitro assays. We show that an intra-REM domain interaction, mediated by phenylalanine 577, is required to allow interaction of the REM domain with the catalytic core, constraining it in the active conformation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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