期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 69, 期 1-2, 页码 15-24出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbbm.2006.02.009
关键词
HPLC; aldehydes; 2,4-dinitrophenylhydrazine; optimization; hexanal
For the study of the derivatization behavior of aliphatic C-1-C-10 aldehydes with 2,4-dinitrophenylhydrazine (DNPH) in a heating block a gradient elution HPLC method for separation and determination by UV detection at 360nm was applied. The influence of time, temperature, excess of reagent and stirring onto the reaction yields were examined for conducting the reaction in a thermostated heating block. First, the derivatization procedure had been screened by experiments according to a complete factorial design in order to evaluate the statistically significant variables. Those parameters were used to establish the optimum conditions for the reaction by means of a Box-Behnken design. (c) 2006 Elsevier B.V. All rights reserved.
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