期刊
BIOCHIMIE
卷 88, 期 12, 页码 1875-1882出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2006.06.017
关键词
Escherichia coli; initiation factor IF1; RNA chaperone; RNA folding; 30S subunit
Translation initiation factor IFI is an indispensable protein for translation in prokaryotes. No clear function has been assigned to this factor so far. In this study we demonstrate an RNA chaperone activity of this protein both in vivo and in vitro. The chaperone assays are based on in vivo or in vitro splicing of the group I intron in the thymidylate synthase gene (td) from phage T4 and an in vitro RNA annealing assay. IFI wild-type and mutant variants with single amino acid substitutions have been analyzed for RNA chaperone activity. Some of the IFI mutant variants are more active as RNA chaperones than the wild-type. Furthermore, both wild-type IFI and mutant variants bind with high affinity to RNA in a band-shift assay. It is suggested that the RNA chaperone activity of IFI contributes to RNA rearrangements during the early phase of translation initiation. (c) 2006 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据